Escherichia coli K-12 substr. MG1655 Polypeptide: arginine export protein

Gene: argO Accession Numbers: EG11159 (EcoCyc), b2923, ECK2919

Synonyms: yggA

Regulation Summary Diagram: ?

Regulation summary diagram for argO

The ArgO (YggA) protein is a member of the LysE family of lysine efflux transporters [Aleshin99]. Based on sequence similarity, ArgO may function as a proton-driven amino acid efflux system.

Null mutations in both the argO and the argP genes cause hypersensitivity to canavanine, an arginine analog. ArgO expression is regulated by ArgP, and transcription of argO is induced by exogenous arginine [Nandineni04].

ArgO = "arginine outward transport" [Nandineni04]

Locations: inner membrane

Map Position: [3,066,195 <- 3,066,830] (66.09 centisomes, 238°)
Length: 636 bp / 211 aa

Molecular Weight of Polypeptide: 23.176 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009591 , EchoBASE:EB1148 , EcoGene:EG11159 , EcoliWiki:b2923 , OU-Microarray:b2923 , PortEco:argO , PR:PRO_000022133 , Pride:P11667 , Protein Model Portal:P11667 , RefSeq:NP_417398 , RegulonDB:EG11159 , String:511145.b2923 , UniProt:P11667

Relationship Links: InterPro:IN-FAMILY:IPR001123 , InterPro:IN-FAMILY:IPR004777 , InterPro:IN-FAMILY:IPR023445 , Panther:IN-FAMILY:PTHR30086 , Pfam:IN-FAMILY:PF01810

Genetic Regulation Schematic: ?

Genetic regulation schematic for argO

GO Terms:

Biological Process: GO:0015809 - arginine transport Inferred from experiment Inferred by computational analysis [GOA01a, Nandineni04]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0015181 - arginine transmembrane transporter activity Inferred by computational analysis [GOA06, GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for argO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Sequence Features

Protein sequence of arginine export protein with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 1 -> 21
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 37 -> 57
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 68 -> 88
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 111 -> 131
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 147 -> 167
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 179 -> 199
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2923 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11159; confirmed by SwissProt match.


Aleshin99: Aleshin VV, Zakataeva NP, Livshits VA (1999). "A new family of amino-acid-efflux proteins." Trends Biochem Sci 1999;24(4);133-5. PMID: 10322417

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Nandineni04: Nandineni MR, Gowrishankar J (2004). "Evidence for an arginine exporter encoded by yggA (argO) that is regulated by the LysR-type transcriptional regulator ArgP in Escherichia coli." J Bacteriol 186(11);3539-46. PMID: 15150242

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bouvier08: Bouvier J, Stragier P, Morales V, Remy E, Gutierrez C (2008). "Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator." J Bacteriol 190(15);5224-9. PMID: 18502871

Goss08: Goss TJ (2008). "The ArgP protein stimulates the Klebsiella pneumoniae gdhA promoter in a lysine-sensitive manner." J Bacteriol 190(12);4351-9. PMID: 18424527

Laishram07: Laishram RS, Gowrishankar J (2007). "Environmental regulation operating at the promoter clearance step of bacterial transcription." Genes Dev 21(10);1258-72. PMID: 17504942

Lee97a: Lee Y, Lee H, Yim J, Hwang D (1997). "The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P." Nucleic Acids Res 1997;25(17);3486-9. PMID: 9254708

Marbaniang11: Marbaniang CN, Gowrishankar J (2011). "Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli." J Bacteriol 193(21);5985-96. PMID: 21890697

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Peeters09: Peeters E, Nguyen Le Minh P, Foulquie-Moreno M, Charlier D (2009). "Competitive activation of the Escherichia coli argO gene coding for an arginine exporter by the transcriptional regulators Lrp and ArgP." Mol Microbiol 74(6);1513-26. PMID: 19906180

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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