Escherichia coli K-12 substr. MG1655 Transporter: L-arginine efflux transporter

Gene: argO Accession Numbers: EG11159 (EcoCyc), b2923, ECK2919

Synonyms: yggA

Regulation Summary Diagram: ?

Regulation summary diagram for argO

ArgO (formerly YggA) is a probable L-arginine efflux transporter in E. coli K-12.

Expression of argO is induced by exogenous L-arginine (or the dipeptide Arg-Ala) in an ArgP dependent manner; expression of argO is reduced by L-lysine (or Lys-Ala). Null mutations in either argO or argP result in hypersensitivity to canavanine, a plant derived arginine analog. Overexpression of argO from a plasmid results in increased L-arginine excretion (in an argR mutant strain that has increased intracellular arginine) [Nandineni04]

ArgO is a member of the LysE family of lysine efflux transporters [Aleshin99].

argO: arginine outward transport" [Nandineni04]

Citations: [Marbaniang12]

Locations: inner membrane

Map Position: [3,066,195 <- 3,066,830] (66.09 centisomes, 238°)
Length: 636 bp / 211 aa

Molecular Weight of Polypeptide: 23.176 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009591 , EchoBASE:EB1148 , EcoGene:EG11159 , EcoliWiki:b2923 , OU-Microarray:b2923 , PortEco:argO , PR:PRO_000022133 , Pride:P11667 , Protein Model Portal:P11667 , RefSeq:NP_417398 , RegulonDB:EG11159 , String:511145.b2923 , UniProt:P11667

Relationship Links: InterPro:IN-FAMILY:IPR001123 , InterPro:IN-FAMILY:IPR004777 , InterPro:IN-FAMILY:IPR023445 , Panther:IN-FAMILY:PTHR30086 , Pfam:IN-FAMILY:PF01810

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for argO

GO Terms:

Biological Process: GO:1902023 - L-arginine transport Inferred from experiment [Nandineni04]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:1903826 - arginine transmembrane transport Inferred by computational analysis [GOA06, GOA01a]
Molecular Function: GO:0015181 - arginine transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, Nandineni04]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Aleshin99]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for argO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Last-Curated ? 18-May-2015 by Mackie A , Macquarie University

Enzymatic reaction of: L-arginine efflux transporter

Transport reaction diagram for L-arginine efflux transporter

Sequence Features

Protein sequence of L-arginine efflux transporter with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 1 -> 21
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 37 -> 57
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 68 -> 88
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 111 -> 131
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 147 -> 167
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 179 -> 199
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2923 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11159; confirmed by SwissProt match.


Aleshin99: Aleshin VV, Zakataeva NP, Livshits VA (1999). "A new family of amino-acid-efflux proteins." Trends Biochem Sci 1999;24(4);133-5. PMID: 10322417

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Marbaniang12: Marbaniang CN, Gowrishankar J (2012). "Transcriptional cross-regulation between Gram-negative and gram-positive bacteria, demonstrated using ArgP-argO of Escherichia coli and LysG-lysE of Corynebacterium glutamicum." J Bacteriol 194(20);5657-66. PMID: 22904281

Nandineni04: Nandineni MR, Gowrishankar J (2004). "Evidence for an arginine exporter encoded by yggA (argO) that is regulated by the LysR-type transcriptional regulator ArgP in Escherichia coli." J Bacteriol 186(11);3539-46. PMID: 15150242

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bouvier08: Bouvier J, Stragier P, Morales V, Remy E, Gutierrez C (2008). "Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator." J Bacteriol 190(15);5224-9. PMID: 18502871

Goss08: Goss TJ (2008). "The ArgP protein stimulates the Klebsiella pneumoniae gdhA promoter in a lysine-sensitive manner." J Bacteriol 190(12);4351-9. PMID: 18424527

Laishram07: Laishram RS, Gowrishankar J (2007). "Environmental regulation operating at the promoter clearance step of bacterial transcription." Genes Dev 21(10);1258-72. PMID: 17504942

Lee97b: Lee Y, Lee H, Yim J, Hwang D (1997). "The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P." Nucleic Acids Res 1997;25(17);3486-9. PMID: 9254708

Marbaniang11: Marbaniang CN, Gowrishankar J (2011). "Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli." J Bacteriol 193(21);5985-96. PMID: 21890697

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Peeters09: Peeters E, Nguyen Le Minh P, Foulquie-Moreno M, Charlier D (2009). "Competitive activation of the Escherichia coli argO gene coding for an arginine exporter by the transcriptional regulators Lrp and ArgP." Mol Microbiol 74(6);1513-26. PMID: 19906180

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.